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ythdc2 human cdna  (OriGene)


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    Structured Review

    OriGene ythdc2 human cdna
    Ythdc2 Human Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2+human+cdna/PCMV6-Neo+Mammalian+Expression+Vector/ppr0403045-65-20-32
    Average 96 stars, based on 456 article reviews
    ythdc2 human cdna - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Over-expression of YTHDC2 Contributes to Progression of Prostate Cancer and Predicts Poor Outcome in Patients with Prostate Cancer
    Article Snippet: Cell culture Four human PCa cell lines (LNCaP, PC-3, DU-145 and 22Rv1) and two immortalized prostate epithelial cell line BPH-1/ RWPE1 were obtained from Guangzhou HYY Med.Co., Ltd and maintained in RPMI 1640 or DMEM supplemented with 10% fetal bovine serum (Thermo Fisher Scienti c, Waltham, MA, USA) and penicillin (50 IU/ml)/streptomycin (50 μg/ml) (Thermo Fisher Scienti c, Waltham, MA, USA). .. YTHDC2-overexpressing/knockdown PCa cell model To construct YTHDC2 overexpression/knockdown stable cells, two PCa cell lines DU145 and PC-3 were transfected with YTHDC2 human cDNA clone or pCMV6-neo vector according to the manufacturer's protocol (Origene, Rockville, MD, USA). .. 48 hours after transfection, cells were selected using G418 (1.2 mg/ml) (Cellgro, Manassas, VA, USA).

    Over Expression:

    Article Title: Over-expression of YTHDC2 Contributes to Progression of Prostate Cancer and Predicts Poor Outcome in Patients with Prostate Cancer
    Article Snippet: Cell culture Four human PCa cell lines (LNCaP, PC-3, DU-145 and 22Rv1) and two immortalized prostate epithelial cell line BPH-1/ RWPE1 were obtained from Guangzhou HYY Med.Co., Ltd and maintained in RPMI 1640 or DMEM supplemented with 10% fetal bovine serum (Thermo Fisher Scienti c, Waltham, MA, USA) and penicillin (50 IU/ml)/streptomycin (50 μg/ml) (Thermo Fisher Scienti c, Waltham, MA, USA). .. YTHDC2-overexpressing/knockdown PCa cell model To construct YTHDC2 overexpression/knockdown stable cells, two PCa cell lines DU145 and PC-3 were transfected with YTHDC2 human cDNA clone or pCMV6-neo vector according to the manufacturer's protocol (Origene, Rockville, MD, USA). .. 48 hours after transfection, cells were selected using G418 (1.2 mg/ml) (Cellgro, Manassas, VA, USA).

    Transfection:

    Article Title: Over-expression of YTHDC2 Contributes to Progression of Prostate Cancer and Predicts Poor Outcome in Patients with Prostate Cancer
    Article Snippet: Cell culture Four human PCa cell lines (LNCaP, PC-3, DU-145 and 22Rv1) and two immortalized prostate epithelial cell line BPH-1/ RWPE1 were obtained from Guangzhou HYY Med.Co., Ltd and maintained in RPMI 1640 or DMEM supplemented with 10% fetal bovine serum (Thermo Fisher Scienti c, Waltham, MA, USA) and penicillin (50 IU/ml)/streptomycin (50 μg/ml) (Thermo Fisher Scienti c, Waltham, MA, USA). .. YTHDC2-overexpressing/knockdown PCa cell model To construct YTHDC2 overexpression/knockdown stable cells, two PCa cell lines DU145 and PC-3 were transfected with YTHDC2 human cDNA clone or pCMV6-neo vector according to the manufacturer's protocol (Origene, Rockville, MD, USA). .. 48 hours after transfection, cells were selected using G418 (1.2 mg/ml) (Cellgro, Manassas, VA, USA).

    Plasmid Preparation:

    Article Title: Over-expression of YTHDC2 Contributes to Progression of Prostate Cancer and Predicts Poor Outcome in Patients with Prostate Cancer
    Article Snippet: Cell culture Four human PCa cell lines (LNCaP, PC-3, DU-145 and 22Rv1) and two immortalized prostate epithelial cell line BPH-1/ RWPE1 were obtained from Guangzhou HYY Med.Co., Ltd and maintained in RPMI 1640 or DMEM supplemented with 10% fetal bovine serum (Thermo Fisher Scienti c, Waltham, MA, USA) and penicillin (50 IU/ml)/streptomycin (50 μg/ml) (Thermo Fisher Scienti c, Waltham, MA, USA). .. YTHDC2-overexpressing/knockdown PCa cell model To construct YTHDC2 overexpression/knockdown stable cells, two PCa cell lines DU145 and PC-3 were transfected with YTHDC2 human cDNA clone or pCMV6-neo vector according to the manufacturer's protocol (Origene, Rockville, MD, USA). .. 48 hours after transfection, cells were selected using G418 (1.2 mg/ml) (Cellgro, Manassas, VA, USA).



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    96
    OriGene ythdc2 human cdna
    Ythdc2 Human Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2+human+cdna/PCMV6-Neo+Mammalian+Expression+Vector/ppr0403045-65-20-32
    Average 96 stars, based on 1 article reviews
    ythdc2 human cdna - by Bioz Stars, 2026-11
    96/100 stars
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